Coding

Part:BBa_K2984050:Design

Designed by: Dimitri Schumacher   Group: iGEM19_Humboldt_Berlin   (2019-10-16)


L1c-PsaDintron-bleRscp-ARS-MHETase-SP20-RbcS2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2614
    Illegal PstI site found at 2938
    Illegal PstI site found at 3281
    Illegal PstI site found at 4091
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2614
    Illegal PstI site found at 2938
    Illegal PstI site found at 3281
    Illegal PstI site found at 4091
    Illegal NotI site found at 2949
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3859
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2614
    Illegal PstI site found at 2938
    Illegal PstI site found at 3281
    Illegal PstI site found at 4091
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2614
    Illegal PstI site found at 2938
    Illegal PstI site found at 3281
    Illegal PstI site found at 4091
    Illegal NgoMIV site found at 1607
    Illegal NgoMIV site found at 2547
    Illegal NgoMIV site found at 3008
    Illegal NgoMIV site found at 3038
    Illegal NgoMIV site found at 3353
    Illegal NgoMIV site found at 3371
    Illegal NgoMIV site found at 3407
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This construct was designed on a level 1 c backbone (BBa_K2984002). Together with L1a (BBa_K2984003) and L1b (BBa_K2984005), a level 2 construct can be built for multiple gene insertion.


Source

The construct was built out of 6 different level 0 plasmids using the MoClo standard (listed in the main description).

References